Mouse

Goat

ELISA

Serum Amyloid A

2-8C

Plasma, Serum

31.25 ng/ml - 2000 ng/ml

11.015 ng/ml

100 min.

The principle of the double antibody sandwich Mouse SAA (serum amyloid a) ELISA is represented in Figure 1. In this assay the SAA present in samples reacts with the anti-Mouse SAA antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Mouse SAA antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound SAA. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of mouse SAA in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of SAA in the test sample. The quantity of SAA in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.